Limberg Jaldin-Crespo completed his Bachelor’s degree in Biochemistry, as one of the top two students of his generation, at the age of 22 years and his Bachelor´s degree in Pharmacy at the age of 25 years, both at the Universidad Autónoma Gabriel René Moreno (UAGRM), Bolivia. He was the Clinical Hematology Laboratory head for 5 years, at the Clinical Laboratory “Dr. Zuna” in Santa Cruz de la Sierra, Bolivia. He earned different scholarships in Clinical Biochemistry, Hematology, Environmental Pollution Management, and Chemical Science in Spain, Argentina, Brazil, and Chile respectively. He earned two Master’s degrees, in Science and Innovation in Medicine and Chemical-Biological Science at the Universidad del Desarrollo (UDD) and Universidad Bernardo O´Higgins (UBO) respectively, both in Chile. Right now, he is a Ph.D. student in Science and Innovation in Medicine, at UDD, Chile.
Wound healing is a global public health problem, and needs urgent therapeutic and research care, particularly in chronic wounds due to delayed healing and bacterial infection susceptibility. The most common causative organisms associated with wound infections include Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. In this study, Chilean honey has been applied to develop green honey-copper nanoparticles (honey-Cu NPs) and evaluate their antimicrobial properties. The synthesized honey-Cu NPs using ascorbic acid, honey, and the assistance of the sonochemical method were characterized by UV-visible, FT-IR, and TSEM techniques. The maximum absorbance was found to be near 600 nm for honey-Cu NP due to surface plasmon resonance. FT-IR spectra confirmed the presence of sugars and proteins in the honey-Cu NPs. The spectral band around 668 and 2900?cm-1 are characteristics of the interaction between Cu and the biomolecules of the honey. TSEM micrographs showed spherical-shaped honey-Cu NPs. ImageJ analysis found the average particle size of the honey-Cu NPs to be 2.8±0,5 nm. It demonstrates that honey has the ability to stabilize and control the size and shape of nanoparticles. The antimicrobial test performed on Escherichia coli ATCC25981 (gram-negative), Pseudomonas aeruginosa ATCC27853 (gram-negative) and Staphylococcus aureus ATCC25923 bacteria (gram-positive) showed a CIM50:73, CIM50:88 and CIM50:45 µg/ml respectively, demonstrating the potential of honey-Cu NPs for wound infectious caused by tested pathogens